Warning: session_start(): Cannot start session when headers already sent in /home/cstore/public_html/config.php on line 46
tiktok takipçiantikaevden eve nakliyateskişehir televizyon tamiriEskişehir uyduığdır evden eve nakliyateskişhir uydu servisidiş eti ağrısıeskişehir emlakgaziantep evden eve nakliyatgaziantep evden eve nakliyatEtimesgut evden eve nakliyatankara evden eve nakliyatmersin evden eve nakliyattuzla evden eve nakliyatvalizgaziantep evden eve nakliyatsakarya evden eve nakliyaten yakın çekiciEtimesgut evden eve nakliyatweb sitesi yapımıgaziantep nakliyat2. el kitap alan yerlerMedyumlarMedyumeskişehir uydu tamirgebze evden eve nakliyatbeykoz evden eve nakliyatmaldives online casinopoodleEtimesgut evden eve nakliyatpoodlepomeranianpancakeswap botdextools trendingdextools trending botdextools botdextools trending servicedextools trending algorithmcoinmarketcap trending botpinksale trending botdextools trending costcoinmarketcap trendingfront run botfront running botpancakeswap sniper botuniswap botuniswap sniper botmev botpinksale trending botprediction botEtimesgut evden eve nakliyatpomeranian ilanlarıMedyummersin evden eve nakliyatseo çalışmasıgoogle adsantika alanlarvozol 12000oto çekicimamak evden eve nakliyatsatılık pomeranian boo ilanlarıpoodlefree hacksbağlama büyüsü
Pharmacology-II Notes(The Cellular level of organisation)Disadvantages of competitive ELISA ELISA advantages and disadvantages ppt

Advantages and Disadvantages of each ELISA type

Bookmark (0)
ClosePlease loginn

No account yet? Register

Advantages Disadvantages
Direct ELISA
  1. Simple protocol, time-saving, and reagents-saving.
  2. No cross-reactivity from secondary antibody.
  1. High background.
  2. No signal amplification, since only a primary antibody is used and a secondary antibody is not needed.
  3. Low flexibility, since the primary antibody must be labeled.
Indirect ELISA
  1. Signal amplification, since one or more secondary antibodies can be used to bind to the primary antibody.
  2. High flexibility, since the same secondary antibody can be used for various primary antibodies.
  1. Complex protocol compared with direct ELISA.
  2. Cross-reactivity from secondary antibody.
Sandwich ELISA
  1. High flexibility.
  2. High sensitivity.
  3. High specificity, since different antibodies bind to the same antigen for detection.
  1. The antigen of interest must be large enough so that two different antibodies can bind to it at different epitopes.
  2. It’s sometimes difficult to find two different antibodies that recognize different epitopes on the antigen of interest and cooperate well in a sandwich format.
Competitive ELISA
  1. High flexibility.
  2. High sensitivity.
  3. Best for the detection of small antigens, even when they are present in low concentrations.
  1. Relatively complex protocol.
  2. Needs the use of inhibitor antigen.
What’s your Reaction?
+1
0
+1
1
+1
1
+1
0
+1
1
+1
0
+1
0

Leave a Comment

Home
Pharm D
Search
Bookmarks
Info

Warning: session_start(): Cannot start session when headers already sent in /home/cstore/public_html/config.php on line 46
deneme bonusu veren -ituder.orgdeneme bonusu veren sitelerdeneme bonusu veren sitelerdenemebonususiteler.comgates of olympus demogates of olympus demobayan escortTürkiye Escort Bayanbuca escortPiabella Giriş TwitterBetgaranti Giriş Twitterİstanbulbahis TwitterMostbet Giriş Twitterfethiye escortmarsbahiscasibom girişizmir escortDenizli escortşanlıurfa escortHacklinkBeşiktaş escortAtaşehir escortBeylikdüzü escortBahis siteleriEsenyurt escort bayanmasöz bayanlarmasöz bayanlarantalya escort bayanlarbetturkeycasibom girişbets10 girişjojobet girişpusulabetbaywin girişGrandpashabet girişcasibom twitterholiganbet güncel girişbettiltcasibom girişslot sitelerisekabetbetmatik girişbetkanyon girişsekabet girişholiganbet girişbetmatikcasibomcasibomcasibom girişcasibom girişcasibom girişcasibom girişcasibom girişcasibom girişcasibom girişhitbet twittersahabet twittersahabet twitterbettilt twittervdcasino twitterilbet twittercratosroyalbet twittertümbet twitterbaywin twitterslot sitelericanlı casino sitelericasino sitelerislot siteleribahis siteleribaywinİnterbahisbelugabahismadridbetkalebetcasibomcasibomcasibom1xbetbahiscombycasinoikimisliorisbetkaçak maç izlecasibomataşehir escortjojobet girişfixbetmatadorbetjojobet girişMeritking