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blood glucose by Glucose oxidase method

Estimation of blood glucose by Glucose oxidase method

Objectives:   To understand the importance of measuring blood glucose level.   To understand the principles of enzymatic estimation of glucose. Theory: Glucose is a simple sugar which is a permanent and immediate primary source of energy to all of the cells in our body. The glucose in blood is obtained from the food that …

Limit test for Lead

Limit test for Lead

Limit test for Lead Lead is a most undesirable impurity in medical compounds and comes through the use of sulphuric acid, lead-lined apparatus and glass bottles use for storage of chemicals. Principle: The limit test of lead is based on the reaction of lead and diphenylthiocabazone (dithizone) in alkaline solution to form lead dithizone complex …

limit test for heavy metal

Limit Test for Heavy Metals

Aim: To perform the limit test for heavy metals for the given sample. Requirements: Nessler cylinders, 1ml bulb pipette, dilute acetic acid, dilute ammonia solution, hydrogen sulphide solution, pH paper: 3-4 range, lead standard solution (20 ppm Pb), glass rods. Principle: In this experiment, the test color obtained by the reaction of heavy metal impurities …

Limit Test for Iron

Limit Test for Iron

Aim: To perform the limit test for iron for the given sample. Requirements: Nessler cylinder, 2ml bulb pipette, 20% w/v iron-free citric acid, thioglycolic acid, ammonia solution, iron standard solution (20 ppm Fe), glass rods, red-litmus paper. Principle: In this experiment, the test color (purple) obtained by the reaction of iron impurities with mercaptoacetic acid …

Limit Test for Sulphates

Limit Test for Sulphates

Aim: To perform limit test for sulphates for the given sample. Requirements: Nessler cylinders, glass rod, 1ml bulb pipette, 2 ml graduate pipette, 5 M acetic acid, sulphate standard solution (10 ppm SO4), test sample, barium chloride solution, ethanolic sulphate standard solution (10 ppm SO4), 1ml graduate pipette. Principle: In this experiment, the test opalescence/turbidity …

Limit Test for Chlorides

Aim: To perform limit test for chlorides for the given sample. Requirements: Nessler cylinders, glass rod, measuring cylinders, 1ml bulb pipette, 10 ml bulb pipette, dilute nitric acid, 0.1 M silver nitrate, chloride standard solution (25 ppm Cl), test sample. Principle: In this experiment, the test opalescence obtained by the reaction of chloride impurities with …

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