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Simple Staining: Principle, Procedure, Uses, Advantages & Disadvantages

Simple Staining: Principle, Procedure, Uses The simple stain can be used as a quick and easy way to determine cell shape, size and arrangements of bacteria. True to its name, the simple stain is a very simple staining procedure involving a single solution of stain. Any basic dye such as methylene blue, safranin, or crystal …

microbiology lab experiments

Instruments used in Microbiology Lab with Principle and Uses

The instruments used in the microbiology labs include a bunch of different kinds of instruments required for a lot of different processes conducted within those laboratories.   List of Instruments used in Microbiology Lab Analytical Balance Autoclave Bunsen burner Centrifuge Colony Counter Deep Freezer Homogenizer Hot plate Hot air oven Incubator Laminar Air Flow/ Laminar …

blood glucose by Glucose oxidase method

Estimation of blood glucose by Glucose oxidase method

Objectives:   To understand the importance of measuring blood glucose level.   To understand the principles of enzymatic estimation of glucose. Theory: Glucose is a simple sugar which is a permanent and immediate primary source of energy to all of the cells in our body. The glucose in blood is obtained from the food that …

Limit test for Lead

Limit test for Lead

Limit test for Lead Lead is a most undesirable impurity in medical compounds and comes through the use of sulphuric acid, lead-lined apparatus and glass bottles use for storage of chemicals. Principle: The limit test of lead is based on the reaction of lead and diphenylthiocabazone (dithizone) in alkaline solution to form lead dithizone complex …

limit test for heavy metal

Limit Test for Heavy Metals

Aim: To perform the limit test for heavy metals for the given sample. Requirements: Nessler cylinders, 1ml bulb pipette, dilute acetic acid, dilute ammonia solution, hydrogen sulphide solution, pH paper: 3-4 range, lead standard solution (20 ppm Pb), glass rods. Principle: In this experiment, the test color obtained by the reaction of heavy metal impurities …

Limit Test for Iron

Limit Test for Iron

Aim: To perform the limit test for iron for the given sample. Requirements: Nessler cylinder, 2ml bulb pipette, 20% w/v iron-free citric acid, thioglycolic acid, ammonia solution, iron standard solution (20 ppm Fe), glass rods, red-litmus paper. Principle: In this experiment, the test color (purple) obtained by the reaction of iron impurities with mercaptoacetic acid …

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