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ELISA ELISA is an antigen-antibody reaction. In 1971, ELISA was introduced by Peter Perlmann and Eva Engvall at Stockholm University in Sweden. It is a common laboratory technique which is usually used to measure the concentration of antibodies or antigens in the blood. ELISA is a plate-based assay technique which is used for detecting and …
Advantages Disadvantages Direct ELISA Simple protocol, time-saving, and reagents-saving. No cross-reactivity from secondary antibody. High background. No signal amplification, since only a primary antibody is used and a secondary antibody is not needed. Low flexibility, since the primary antibody must be labeled. Indirect ELISA Signal amplification, since one or more secondary antibodies can be used …
Erythropoiesis(Production of Red Blood Cells) Hematopoiesis describes the production of cells that circulate in the bloodstream. Specifically, erythropoiesis is the process by which red blood cells (erythrocytes) are produced. On average, the body produces an astounding 2.5 billion red cells/kg/day. Erythrocytes arise from a complex line of cells, and their rate of production is tightly regulated …
DIABETES Diabetes mellitus (DM), commonly known as diabetes, is a group of metabolic disorders characterized by a high blood sugar level over a prolonged period of time. This is a disease that occurs when your body doesn’t make or use the hormone insulin properly. It causes too much blood glucose (sugar) to build up in …
The Cell Cells are the smallest functional units of the body. They are grouped together to form tissues, each of which has a specialised function. e.g. blood, muscle, bone. Different tissues are grouped together to form organs. e.g. heart, stomach, brain. Organs are grouped together to form systems. e.g. the digestive system is responsible for …