The enzyme was extracted from Aspergillus niger by growing it in Czapecl dox media broth and filtration followed by centrifugation. Measurement of activity showed 0.342 µ mol/min in polygalacturonase DNS assay. an experiment was done with 5ml broccoli juice and enzyme added at the rate of 4-40% v/v for clarification. Lower values not giving satisfactory results. Is this combination possible? Looking for answers urgently. Kindly help.