Medicinal Biochemistry

Click Here to Download Notes: Biomolecules: Introduction Part – 1

Reviewed By: Vishwjeet Singh Click Here to Download Hand Written Notes on Radio Immuno Assay(RIA)

ELISA ELISA is an antigen-antibody reaction. In 1971, ELISA was introduced by Peter Perlmann and Eva Engvall at Stockholm University in Sweden. It is a common laboratory technique which is usually used to measure the concentration of antibodies or antigens in the blood. ELISA is a plate-based assay technique which is used for detecting and …

Advantages Disadvantages Direct ELISA Simple protocol, time-saving, and reagents-saving. No cross-reactivity from secondary antibody. High background. No signal amplification, since only a primary antibody is used and a secondary antibody is not needed. Low flexibility, since the primary antibody must be labeled. Indirect ELISA Signal amplification, since one or more secondary antibodies can be used …

DIABETES Diabetes mellitus (DM), commonly known as diabetes, is a group of metabolic disorders characterized by a high blood sugar level over a prolonged period of time. This is a disease that occurs when your body doesn’t make or use the hormone insulin properly. It causes too much blood glucose (sugar) to build up in …