Answers

Enzymes (/ˈɛnzaɪmz/) are proteins that act as biological catalysts (biocatalysts). Catalysts accelerate chemical reactions. The molecules upon which enzymes may act are called substrates, and the enzyme converts the substrates into different molecules known as products

Alkaline hydrolysis of primary alkyl halides is one of the best examples of SN2 reaction. In this ,nucleophile attacks from back side of the substrate , i.e. side opposite to the leaving group . Hence attachment of nucleophile and separation of leaving group takes place simultaneously to form transition state. further attack of nucleophile causes …

The enzyme was extracted from Aspergillus niger by growing it in Czapecl dox media broth and filtration followed by centrifugation. Measurement of activity showed 0.342 µ mol/min in polygalacturonase DNS assay. an experiment was done with 5ml broccoli juice and enzyme added at the rate of 4-40% v/v for clarification. Lower values not giving satisfactory …