Alkaline hydrolysis of primary alkyl halides is one of the best examples of SN2 reaction. In this ,nucleophile attacks from back side of the substrate , i.e. side opposite to the leaving group . Hence...
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The enzyme was extracted from Aspergillus niger by growing it in Czapecl dox media broth and filtration followed by centrifugation. Measurement of activity showed 0.342 µ mol/min in polygalacturonase...
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