The addition of HBr to unsymmetrical alkenes against the Markownikoff s rule is called peroxide effect or Anti Markownikoff s rule. According to this effect, “the negative part of the unsymmetrical attacking r […]
The enzyme was extracted from Aspergillus niger by growing it in Czapecl dox media broth and filtration followed by centrifugation. Measurement of activity showed 0.342 µ mol/min in polygalacturonase DNS assay. […]